Genomics Core Facility at MCB - a modernly equipped unit, staffed with experienced technicians and scientists dedicated to genome variation studies - offers its services in the area of next-generation sequencing and capillary electrophoresis to all interested researchers and institutions. We believe that our expertise combined with befitting infrastructure will let us assure the best quality of rendered services and the output data.
Next-generation sequencing (NGS), also known as massively parallel or high-throughput DNA sequencing, is a term describing novel DNA sequencing technologies which have revolutionized genomic research in the last decades. Because its unprecedented speed and throughput allow us to study DNA variation on a much larger scale than ever before, NGS became an indispensable tool helping to address complex biological problems.
At present, we carry out high-throughput DNA sequencing experiments utilizing two Ion Torrent platforms (PGM and Proton) and the MinION sequencer from Oxford Nanopore Technologies. The Ion Torrent technology allow us to offer a range of NGS applications and the MinION gives us some extra possibilities like sequencing of ultra long products (hundreds of kb) or performing native sequencing (without base modifications), e.g. for the DNA methylation analysis or direct RNA sequencing.
Capillary electrophoresis (CE) is a method commonly used for DNA analysis, as it allows rapid electrophoretic separations for various applications. The MCB‘s Genomics Core Facility offers CE services utilizing the 3500 Series Genetic Analyzer manufactured by Applied Biosystems. The instrument can be used for examination of products of Sanger sequencing and DNA fragment analysis, which is applied in a number of genetic assays (e.g. microsatellite genotyping, MLPA, AFLP, LOH).
To obtain more information about our services, request a quote or place an order, please contact us:
Prof. Wojciech Branicki
Tel.: +48 12 664 61 11
MSc. Agata Jarosz
Tel.: +48 12 664 61 13