International Asssociated Laboratory - Laboratoire International Associé (LIA)

Group leader

prof. dr hab. Józef Dulak,
Head of Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology

Research area

Joint laboratory of the Faculty of Biochemistry, Biophysics and Biotechnology & Malopolska Centre of Biotechnology with the CNRS in Orleans, France

This joint laboratory (“without walls”) has been established in 2013 upon the official agreement signed between the Jagiellonian University and CNRS. This year the prolongation of the cooperation for the next four years (2017-2020) will be signed. The Polish and French research groups will cooperate in the frame of the activities entitled “Biomarkers and mediators of diseases”

Department’s website

Grants (partially) conducted at MCB

  • The National Science Centre - OPUS Programme - Impaired angiogenesis in Duchenne muscular dystrophy - is there a role for heme oxygenase-1 and statins? 2017-2019 Principal investigator – Agnieszka Łoboda
  • The National Centre Research and Development - STRATEGMED Programme - Epigenetic Therapies in Oncology - EPTHERON consortium, 2015-2017
  • The National Centre Research and Development - Applied Research Programme - New inhibitors of heme oxygenase-1 pathway as the potential anticancer drugs - in cooperation with Selvita S.A, HemOx 2014-2017

Publications with MCB affiliation:

[1] K. Klimkiewicz, K. Weglarczyk, G. Collet, M. Paprocka, A. Guichard, M. Sarna, A. Jozkowicz, J. Dulak, T. Sarna, C. Grillon, C. Kieda, A 3D model of tumour angiogenic microenvironment to monitor hypoxia effects on cell interactions and cancer stem cell selection, Cancer Lett. 396 (2017) 10–20. doi:10.1016/j.canlet.2017.03.006.

[2] J. Ligeza, P. Marona, N. Gach, B. Lipert, K. Miekus, W. Wilk, J. Jaszczynski, A. Stelmach, A. Loboda, J. Dulak, W. Branicki, J. Rys, J. Jura, MCPIP1 contributes to clear cell renal cell carcinomas development, Angiogenesis. (2017). doi:10.1007/s10456-017-9540-2.

[3] K. Szade, M. Zukowska, A. Szade, G. Collet, D. Kloska, C. Kieda, A. Jozkowicz, J. Dulak, Spheroid-plug model as a tool to study tumor development, angiogenesis, and heterogeneity in vivo, Tumour Biol. 2016 Feb;37(2):2481-96. doi:10.1007/s13277-016-5099-6.

[4] A. Jazwa, U. Florczyk, A. Grochot-Przeczek, B. Krist, A. Loboda, A. Jozkowicz, J. Dulak, Limb ischemia and vessel regeneration: Is there a role for VEGF?, Vascul. Pharmacol. 86 (2016) 18–30. doi:10.1016/j.vph.2016.09.003.

[5] N. Kachamakova-Trojanowska, W. Nowak, K. Szade, J. Stepniewski, K. Bukowska-Strakova, M. Zukowska, H. Taha, A. Chmura-Skirlinska, M. Beilharz, J. Dulak, A. Jozkowicz, Generation of functional endothelial cells with progenitor-like features from murine induced pluripotent stem cells, Vascul. Pharmacol. 86 (2016) 94–108. doi:10.1016/j.vph.2016.07.008.

[6] M. Ciesla, P. Marona, M. Kozakowska, M. Jez, M. Seczynska, A. Loboda, K. Bukowska-Strakova, A. Szade, M. Walawender, M. Kusior, J. Stepniewski, K. Szade, B. Krist, O. Yagensky, A. Urbanik, B. Kazanowska, J. Dulak, A. Jozkowicz, Heme Oxygenase-1 Controls an HDAC4-miR-206 Pathway of Oxidative Stress in Rhabdomyosarcoma, Cancer Res. 76 (2016) 5707–5718. doi:10.1158/0008-5472.CAN-15-1883.

[7] M. Kozakowska, B. Dobrowolska-Glazar, K. Okoń, A. Józkowicz, Z. Dobrowolski, J. Dulak, Preliminary Analysis of the Expression of Selected Proangiogenic and Antioxidant Genes and MicroRNAs in Patients with Non-Muscle-Invasive Bladder Cancer, J. Clin. Med. 5 (2016). doi:10.3390/jcm5030029.

[8] A. Loboda, M. Damulewicz, E. Pyza, A. Jozkowicz, J. Dulak, Role of Nrf2/HO-1 system in development, oxidative stress response and diseases: an evolutionarily conserved mechanism, Cell. Mol. Life Sci. CMLS. 73 (2016) 3221–3247. doi:10.1007/s00018-016-2223-0.

[9] M.A. Papież, W. Krzyściak, K. Szade, K. Bukowska-Straková, M. Kozakowska, K. Hajduk, B. Bystrowska, J. Dulak, A. Jozkowicz, Curcumin enhances the cytogenotoxic effect of etoposide in leukemia cells through induction of reactive oxygen species, Drug Des. Devel. Ther. 10 (2016) 557–570. doi:10.2147/DDDT.S92687.

[10] M. Kozakowska, K. Pietraszek-Gremplewicz, A. Jozkowicz, J. Dulak, The role of oxidative stress in skeletal muscle injury and regeneration: focus on antioxidant enzymes, J. Muscle Res. Cell Motil. 36 (2015) 377–393. doi:10.1007/s10974-015-9438-9.

[11] M. Bak, A. Jazwa, L. Kasper, N. Kachamakova-Trojanowska, A. Jozkowicz, K. Sladek, J. Dulak, Involvement of microRNAs in the inflammatory pathways of pulmonary sarcoidosis, J. Physiol. Pharmacol. Off. J. Pol. Physiol. Soc. 66 (2015) 635–642.

[12] G. Collet, K. Szade, W. Nowak, K. Klimkiewicz, B. El Hafny-Rahbi, K. Szczepanek, D. Sugiyama, K. Weglarczyk, A. Foucault-Collet, A. Guichard, A. Mazan, M. Nadim, F. Fasani, N. Lamerant-Fayel, C. Grillon, S. Petoud, J.-C. Beloeil, A. Jozkowicz, J. Dulak, C. Kieda, Endothelial precursor cell-based therapy to target the pathologic angiogenesis and compensate tumor hypoxia, Cancer Lett. 370 (2016) 345–357. doi:10.1016/j.canlet.2015.11.008.

[13] M. Tertil, S. Golda, K. Skrzypek, U. Florczyk, K. Weglarczyk, J. Kotlinowski, M. Maleszewska, S. Czauderna, C. Pichon, C. Kieda, A. Jozkowicz, J. Dulak, Nrf2-heme oxygenase-1 axis in mucoepidermoid carcinoma of the lung: Antitumoral effects associated with down-regulation of matrix metalloproteinases, Free Radic. Biol. Med. 89 (2015) 147–157. doi:10.1016/j.freeradbiomed.2015.08.004.

[14] A. Loboda, A. Jozkowicz, J. Dulak, HO-1/CO system in tumor growth, angiogenesis and metabolism - Targeting HO-1 as an anti-tumor therapy, Vascul. Pharmacol. 74 (2015) 11–22. doi:10.1016/j.vph.2015.09.004.

[15] K. Szade, M. Zukowska, A. Szade, G. Collet, D. Kloska, C. Kieda, A. Jozkowicz, J. Dulak, Spheroid-plug model as a tool to study tumor development, angiogenesis, and heterogeneity in vivo, Tumour Biol. J. Int. Soc. Oncodevelopmental Biol. Med. 37 (2016) 2481–2496. doi:10.1007/s13277-015-4065-z.

[16] M. Kozakowska, J. Kotlinowski, A. Grochot-Przeczek, M. Ciesla, B. Pilecki, R. Derlacz, J. Dulak, A. Jozkowicz, Myoblast-conditioned media improve regeneration and revascularization of ischemic muscles in diabetic mice, Stem Cell Res. Ther. 6 (2015) 61. doi:10.1186/s13287-015-0063-8.

[17] A. Jazwa, M. Stoszko, M. Tomczyk, K. Bukowska-Strakova, C. Pichon, A. Jozkowicz, J. Dulak, HIF-regulated HO-1 gene transfer improves the post-ischemic limb recovery and diminishes TLR-triggered immune responses - Effects modified by concomitant VEGF overexpression, Vascul. Pharmacol. 71 (2015) 127–138. doi:10.1016/j.vph.2015.02.011.

18] A. Szade, W.N. Nowak, K. Szade, A. Gese, R. Czypicki, H. Waś, J. Dulak, A. Józkowicz, Effect of crossing C57BL/6 and FVB mouse strains on basal cytokine expression, Mediators Inflamm. 2015 (2015) 762419. doi:10.1155/2015/762419.

[19] Szade A, Grochot-Przeczek A, Florczyk U, Jozkowicz A, Dulak J. Cellular and molecular mechanisms of inflammation-induced angiogenesis. IUBMB Life 2015; 67: 145-159. doi: 10.1002/iub.1358.

[20 J. Stepniewski, N. Kachamakova-Trojanowska, D. Ogrocki, M. Szopa, M. Matlok, M. Beilharz, G. Dyduch, M.T. Malecki, A. Jozkowicz, J. Dulak, Induced pluripotent stem cells as a model for diabetes investigation, Sci. Rep. 5 (2015) 8597. doi:10.1038/srep08597.

[21] Loboda A, Jozkowicz A, Dulak J. Carbon monoxide: pro- or anti-angiogenic agent? Comment on Ahmad et al. (Thromb Haemost 2015; 113: 329-337). Thromb Haemost. 2015; 114: 432-433. doi: 10.1160/TH15-01-0082.