Laboratorium Biomateriałów i Komórek Macierzystych

Group Leader

Marek Łos, PhD, DSc, email: bioappl@gmail.com

The group is interested in topic on the boundary of regenerative medicine and cancer. It uses reprogramming not only for regenerative-medicine purposes, but also as a model of carcinogenesis.

Researched topics include:

  • anticancer therapeutics preferentially/selectively targeting cancer (stem) cells 
  • differences between normal and cancer stem cells
  • reprogramming-associated carcinogenesis
  • development and optimization of novel transdifferntiation methods
  • interactions between cells and biomaterials, under normal-, hypoxic and electrostimulated conditions
  • development of prosthetics/tissue-replacements required in surgical procedures as required by collaboration partners
  • among biochemical processes studied the group currently investigates apoptosis, autophagy, necroptosis and mitophagy under various pathophysiologic conditions

The research is conducted in multinational collaborations that currently involve Polish, Canadian, Iranian, French, and Nordic universities.

Marek Łos Publications

Hirsch-Factor: 48 Average citation per article: ~67, Cited 9000 times 5-year-journals’ Impact Factors based on 2015.

A. Original research papers 5-year ISI-IF/citations in late Aug.-16

1 Los, M., Dröge, W. and K. Schulze-Osthoff, (1994) Inhibition of AP-1 activation by CD28 signaling. Biochem. J. 302: 119-123. 4,1/6

2 Los, M., Dröge, W., Stricker K., Baeuerle, P.A. and K. Schulze-Osthoff (1995) Hydrogen peroxide as a potent activator of T lymphocyte functions. Eur. J. Immunol. 25: 159-165. 4,3/160

3 Westendorp, M., Shatrov, V., Schulze-Osthoff, K., Frank, R., Kraft, M., Los, M., Krammer, P.H., Dröge, W. and V. Lehmann, (1995) HIV-1 Tat potentiates TNF-induced NF-kB activation and cytotoxicity by altering the cellular redox state. EMBO J. 14: 546-554. 9,3/315

4 Los, M., van de Craen, M., Penning, L., Schenk, H., Westendorp, M., Baeuerle, P.A., Dröge, W., Krammer, P.H. Fiers, W. and K. Schulze-Osthoff, (1995) Requirement of an ICE/Ced-3 protease for Fas/APO-1-mediated apoptosis. Nature 375: 81-83. 41,4/631

5 Los, M., Schenk, H., Hexel, K., Baeuerle, P.A., Dröge, W. and K. Schulze-Osthoff, (1995) IL-2 gene expression and NF-kB activation through CD28 requires reactive oxygen production by 5-Lipoxygenase. EMBO J. 14: 3731.3740. 9,3/247

6 Wang, Z-Q., Stingl, L., Morrison, C., Jantsch, M., Los, M., Schulze-Osthoff, K. and E.F. Wagner, (1997) PARP is important for genomic stability but dispensible in apoptosis. Genes & Dev. 11: 2347-2358. 11,6/420

7 Grage-Griebenow, E., Baran, J., Loppnow, H., Los, M., Ernst, M., Flad, H-D. and J. Pryjma, (1997) An Fc-gamma receptor I (CD64) negative subpopulation of human peripheral blood monocytes is resistant to killing by antigen-activated CD4-positive cytotoxic T cells. Eur. J. Immunol. 27: 2358-2365. 4,3/12

8 Los, M., Herr, I., Friesen, C., Fulda, S., Schulze-Osthoff, K. and K.M. Debatin, (1997) Cross-Resistance of CD95- and Drug-Induced Apoptosis as a Consequence of Deficient Activation of Caspases. Blood 90: 3118-3129. 10,2/161

9 Fulda, S., Los M., Mier, W. and K.M. Debatin, (1997) Betulinic acid triggers CD95 (APO-1/Fas)- and p53-independent apoptosis via activation of caspases in neuroectodermal tumors. Cancer Res. 57: 4956-4964. 9,2/265

10 Bauer, M.K.,Vogt, M., Los, M., Siegel, J., Wesselborg, S. and K. Schulze-Osthoff, (1998) Role of Reactive Oxygen Intermediates in Activation-induced CD95 (APO-1/Fas) Ligand Expression. J. Biol. Chem. 273: 8048-8055. 4,4/144

11 Ferrari, D., Stepczynska, A., Los, M., Wesselborg, S. and K. Schulze-Osthoff (1998) Differental regulation of caspase-8 and -3 activation during CD95- and anticancer drug-induced apoptosis. J. Exp. Med. 188: 979-84. 13/168

12 Fulda, S., Los, M., Friesen, C. and K.M. Debatin, (1998) Chemosensitivity of solid tumor cells in vitro is related to activation of the CD95 system. Int. J. Cancer, 76: 105-114. 5,6/140

13 Los, M., Khazaie, K., Schulze-Osthoff, K., Baeuerle, P.A., Schirrmacher, V. and K. Chlichlia (1998) Human T cell leukemia virus-I (HTLV-I) Tax-mediated T-cell apoptosis follows an enhanced intracellular prooxidant state. J. Immunol. 161: 3050-5. 5,3/199

14 Wesselborg, S., Engels, I.H., Rossmann, E., Stepczynska, A., Los, M. and K. Schulze-Osthoff, (1999) Anticancer drugs induce caspase-8 (FLICE) activation through a mechanism not requiring death receptor/ligand interaction. Blood, 93: 3053-63. 10,2/256

15 Ferrari, D., Los, M., Bauer, M.K., Vandenabeele, P., Wesselborg, S. and K. Schulze-Osthoff (1999) P2Z purinoreceptor ligation induces activation of caspases with distinct roles in apoptotic and necrotic alterations of cell death. FEBS Lett. 447: 71-5. 3,5/199

16 Hug, H., Los, M., Hirt, W. and K-M. Debatin (1999) Rhodamine 110-linked aminoacids and peptides as substrates to measure caspase activity upon apoptosis induction in intact cells. Biochemistry 19: 13906-13911. 2,8/94

17 Belka, C., Marini, P., Lepple-Wienhues, A., Jekle, A., Los, M., Schulze-Osthoff, K., Gulbins, E. and M. Bamberg (1999) The tyrosine kinase Lck is required for CD95 independent caspase8 activation and apoptosis in response to ionising radiation. Oncogene 2: 4983-92. 7,4/74

18 Los, M., Neubüser, D., Coy, J.F., Mozoluk, M., Poustka, A. and K. Schulze-Osthoff (2000) Functional characterization of DNase X, a novel apoptotic endonuclease expressed in muscle cells. Biochemistry 27: 7365-73. 2,8/30

19 Renz, A., Burek, C., Mier, W., Mozoluk, M., Schulze-Osthoff, K. and M. Los, (2001) Cytochrome c is rapidly extruded from apoptotic cells and detectable in serum of anticancer-drug treated tumor patients. Adv. Exp. Med. Biol. 495: 331-4. 1,8/13

20 Burek, C., Roth, J., Koch, H-G., Harzer, K., Los, M.* and K. Schulze-Osthoff, (2001) The role of ceramide in receptor- and stress-induced apoptosis studied in acidic ceramidase-deficient Farber disease cells. Oncogene 20: 6493-502. 7,4/29

21 Renz, A., Berdel, W.E., Kreuter, M., Belka, C., Schulze-Osthoff, K. and M. Los, (2001) Rapid extracellular release of cytochrome c is specific for apoptosis and marks cell death in vivo. Blood, 98: 1542-8. 10,2/103

22 Los, M., Mozoluk, M., Ferrari, D., Stepczynska, A., Renz, A., Stroh, C., Herceg, Z., Wang, Z-Q. and K. Schulze-Osthoff, (2002) Activation and caspase-mediated inhibition of PARP: a molecular switch between fibroblast necrosis and apoptosis in death receptor signaling. Mol. Biol. Cell, 13:978-88. 4,3/210

23 Stroh, C., Cassens, U., Samraj, A.K., Sibrowski, W., Schulze-Osthoff, K. and M. Los, (2002) The role of caspases in cryoinjury: caspase inhibition strongly improves the recovery of cryopreserved hematopoietic and other cells FASEB J. 16:1651-3. 5,5/57

24 Burek, C.J., Burek, M., Roth, J. and M. Los, (2003) Calcium induces apoptosis and necrosis in hematopoetic malignant cells: evidence for caspase-8 dependent, FADD-autonomous and Bcl-2-inhibitable pathway. Gene Ther. Mol. Biol. 7:173-9. 0,8/

25 Baust, H., Schoke, A., Brey, A., Gern, U., Los, M., Schmid, R.M., Röttinger, E.M. and T. Seufferlein (2003) Evidence for radiosensitizing by gliotoxin in HL-60 cells: implications for a role of NF-B independent mechanisms. Oncogene 22:8786-96. 7,4/6

26 Ghavami, S., Kerkhoff, C., Los, M., Hashemi, M., Sorg, C. and F. Karami-Tehrani (2004) Mechanism of apoptosis induced by S100A8/A9 in colon cancer cell lines: The role of ROS and the effect of metal ions. J. Leukoc. Biol. 76:169-75. 4,5/87

27 Barczyk, K., Kreuter, M., Pryjma, J., Booy, E.P., Maddika, S., Ghavami, S., Berdel, W.E., Roth, J. and M. Los (2005) Serum cytochrome c indicates in vivo apoptosis and can serve as a prognostic marker during cancer therapy Int. J. Cancer 116:167-73. 5,6/61

28 Hashemi, M., Karami-Tehrani, F., Ghavami, S., Maddika, S. and M. Los. (2005) Adenosine and deoxyadenosine induces apoptosis in estrogen receptor positive and negative human breast cancer cells via the intrinsic pathway. Cell Proliferat. 38:269-85. 3,1/25

29 Maddika, S., Booy, E.P., Johar, D., Gibson, S.B., Ghavami, S. and M. Los (2005) Cancer-specific toxicity of apoptin is independent of death receptors but involves the loss of mitochondrial membrane potential and the release of mitochondrial cell death mediators by a Nur77-dependent pathway. J. Cell Sci. 118:4485-93. 5,5/70

30 Burek, M., Maddika, S., Burek, C. Daniel, P.T., Schulze-Osthoff, K. and M. Los. (2006) Apoptin-induced cell death is modulated by Bcl-2 family members and is Apaf-1-dependent. Oncogene 25:2213-22. 7,4/35

31 Grote, J., König, S., Ackermann, D., Sopalla, C., Benedyk, M., Los, M. and Kerkhoff C. (2006) Identification of poly(ADP-ribose)polymerase-1 and Ku70/Ku80 as transcriptional regulators of S100A9 gene expression. BMC Molecular Biology 7:48. 2,7/20

32 Hashemi, M., Ghavami, S., Eshraghi, M., Booy, E.P., and M. Los, (2007), Cytotoxic effects of intra- and extracellular zinc chelation on human breast cancer cells. Eur. J. Pharm., 557:9–19. 2,7/68

33 Maddika, S., Bay, G.H., Kroczak, T.J., Ande, S.R., Maddika, S., Wiechec, E., Gibson, S.B., and M. Los (2007) Akt is transferred to the nucleus of cells treated with apoptin, and it participates in apoptin-induced cell death. Cell Proliferat. 40:835-848. 3,1/34

34 Zuse, A., Prinz, H,. Müller, K., Schmidt, P., Günther, E.G., Schweizer, F., Prehn, J.H.M. and M. Los (2007) 9-Benzylidene-naphtho[2,3 b]thiophen-4-ones and Benzylidene-9(10H)-anthracenones as novel tubulin interacting agents with high apoptosis-inducing activity. Eur. J. Pharm. 575:34-45. 2,7/13

35 Ghavami, S., Kerkhoff, C., Chazin, W.J., Kadkhoda, K., Xiao, W., Zuse, A., Hashemi, M., Eshraghi, M., Schulze-Osthoff, K., Klonisch, T. and M. Los. (2008) S100A8/9 Induces Cell Death via a Novel, RAGE-independent Pathway that Involves Selective Release of Smac/Diablo and Omi/HtrA2 BBA Mol. Cell. Res. 1783: 297–311. 5,3/68

36 Maddika, S., Ande, S.R., Wiechec, E., Hansen, L.L., Wesselborg, S. and M. Los, (2008) Akt mediated phosphorylation of CDK2 regulates its dual role in cell cycle and apoptosis. J. Cell Sci. 121:979-88. 5,5/76

37 Ghavami, S., Asoodeh, A., Klonisch, T., Halayko, A.J., Kadkhoda, K., Kroczak, T.J., Gibson, S.B., Booy, E.P., Naderi-Manesh, H., and M. Los (2008) Brevinin-2R semi-selectively kills cancer cells by a distinct mechanism which involves the lysosomal-mitochondrial death pathway. J. Cell. Mol. Med. 12:1005-1022. 4,6/71

38 Ghavami, S., Rashedi, I., Dattilo, B.M., Eshraghi, M., Chazin, W.J., Hashemi, M., Wesselborg, S., Kerkhoff, C. and M. Los. (2008) S100A8/A9 at low concentration promotes tumor cell growth via RAGE ligation and MAP kinase-dependent pathway. J. Leukoc. Biol. 83:1484-1492. 4,5/151

39 Maddika, S., Wiechec, E., Ande, S.R., Poon, I.K., Fischer, U., Wesselborg, S., Jans, D.A., Schulze-Osthoff, K. and M. Los, (2008) Interaction with PI3 kinase contributes to the cytotoxic activity of Apoptin. Oncogene 27:3060-5. 7,4/31

40 Glogowska, A., Pyka, J,. Kehlen, A., Los, M., Perumal, P., Weber, E., Dralle, H., Cheng, S-Y., Hoang-Vu, C. and T. Klonisch. (2008) The cytoplasmic domain of proEGF negatively regulates motility and elastinolytic activity in thyroid carcinoma cells. Neoplasia 10:1120-30. 4,8/13

41 Alberti, E.A., Los, M., García, R., Fraga, J.L., Serrano, T., Hernández, E., Klonisch, T., Macías, R., Martínez, L., Castillo, L. and C. de la Cuétara. (2009) Prolonged survival and expression of neural markers by bone marrow-derived stem cells transplanted into brain lesions. Med. Sci. Monit. 15:BR47-54. 1,3/15

42 Ghavami, S., Eshraghi, M., Kadkhoda, K., Mutawe, M.M., Maddika, S., Bay, G.H., Wesselborg, S., Halayko, A.H., Klonisch, T. and M. Los. (2009) Role of BNIP3 in Tumor Necrosis Factor Alpha-induced-cell death – TNF upregulates BNIP3 expression. BBA Mol. Cell. Res. 1783:546–560. 5,3/23

43 Maddika, S., Panigrahi, S., Wiechec, E., Wesselborg, S., Fischer, U., Schulze-Osthoff, K. and M. Los. (2009) Unscheduled Akt-triggered activation of CDK2 as a key effector mechanism of apoptin's anticancer toxicity. Mol. Cell Biol. 29:1235–1248. 4.8/48

44 Ghavami, S., Eshragi, M., Ande, S.R., Chazin, W.J, Klonisch, T., Halayko, A.J., Hashemi, M., Kerkhoff, C. and M. Los. (2010) S100A8/A9 induces autophagy and apoptosis via ROS mediated cross-talk between mitochondria and lysosomes. Cell Res. 20:314-31. 12,4/75

45 Ghavami, S., Mutawe, M.M., Hauff, K., Stelmack, G.L., Schafssma, D., Sharma, P., McNeill, K.D., Hynes, H., Kung, S.K., Unruh, H., Klonisch, T., Hatch, G., Los, M., and A.J. Halayko. (2010) Statin-triggered cell death in primary human lung mesenchymal cells involves p53-PUMA and release of Smac and Omi but not cytochrome c. BBA Mol. Cell. Res., 1803:452–467. 5,3/35

46 Gökay, O., Kühner, D., Los, M., Bertsche, U., Götz, F., Albert, K. (2010) A new Approach for the Rapid Preparation, Isolation, Evaluation and Identification of Antimicrobial Plant Components Demonstrated on the Example Radix Imperatoriae. Anal. Bioanal. Chem., 398:2039-

47. 3,3/8 47 Ghavami, S., Mutawe, M., Sharma, P., Yeganeh, B., McNeill, K.D., Klonisch, T., Unruh, H., Kashani HH., Schaafsma, D., Los, M., Halayko, A.J. (2011) Mevalonate cascade regulation of airway mesenchymal cell autophagy and apoptosis: a dual role for p53. PLoS One, 6:e16523. 3,5/42

48 Ghavami, S., Cunnington, R.H., Yeganeh, B., Davies, J.J.L., Rattan, S.G., Bathe, K, Kavosh, M., Los, M.J., Freed, D.H., Klonisch, T., Pierce, G.N., Halayko, A.J., and I.M.C. Dixon. (2012) Autophagy Regulates Trans Fatty Acid-Mediated Apoptosis in Primary Cardiac Myofibroblasts, BBA Mol. Cell. Res., 1823:2274-86. 5,3/14

49 Panigrahi, S., Stetefeld, J., Jangamreddy J.R., Mandal, S., Mandal, S.K. and M. Los. (2012) Modeling of molecular interaction between Apoptin, BCR-Abl and Crkl - an alternative approach to conventional rational drug design. PLoS One, 7:e28395. 3,5/10

50 Hashemi, M., Fazaeli, A., Eskandari-Nasab, E., Arbabi, F., Mashhadi, M.A., Taheri, M., Ghavami, S., Chaabane, W., Jain. M.V., and M. Los. (2013) Functional polymorphisms of FAS and FASL gene and risk of breast cancer – pilot study of 134 cases. PLoS One, 8(1):e53075. DOI: 10.1371/journal.pone.0053075). 3,5/36

51 Jangamreddy J.R., Ghavami, S., Grabarek, J., Kratz, G., Wiechec E., Fredriksson, B.A., Rao R.K., Cieślar-Pobuda, A., Panigrahi, S., M.J. Los. (2013) Salinomycin induced activation of autophagy, mitophagy, and affects mitochondrial polarity: differences between primary-, and cancer cells BBA Mol. Cell. Res., 1833:2057–69. 5,3/34

52 Kumar, M.D., Yamaguchi, M., Miura, K., Los, M., Coy, J.F., and Y. Rikihisa. (2013) DNase X, a GPI-anchored nucleotidase, Is a Mammalian Receptor for Entry for the Novel Ehrlichia chaffeensis Invasin ECH1038" PLoS Pathog., 9(10):e1003666 7,8/8

53 Cieślar-Pobuda, A., Back, M., Magnusson, K., Jain, M.V., Rafat, M., Ghavami, S., Nilsson, K.P.R., and M.J. Łos. (2014) Cell type related differences in staining with pentameric thiophene derivatives Cytometry A, 85:628-35. 3,2/8

54 Ghavami S, Sharma P, Yeganeh B, Ojo OO, Jha A, Mutawe MM, Kashani HH, Los MJ, Klonisch T, Unruh H, Halayko AJ. (2014) Airway Mesenchymal Cell Death by Mevalonate Cascade Inhibition: Integration of autophagy, unfolded protein response and apoptosis focusing on Bcl2 family proteins. BBA Mol. Cell. Res., 1843:1259-71. 5,3/13

55 Jangamreddy JR, Panigrahi S, Lotfi K, Yadav M, Maddika S, Tripathi AK, Sanyal S, and MJ Łos, (2014) Mapping of Apoptin-interaction with BCR-ABL1, and development of apoptin-based targeted therapy Oncotarget, 5:7198-211. 5,4/2

56 Chaabane W, Cieślar-Pobuda A, El-Gazzah M, Jain MV, Rzeszowska-Wolny J, Rafat M, Stetefeld J, Ghavami S, and MJ Łos (2014), Human-Gyrovirus-Apoptin Triggers Mitochondrial Death Pathway - Nur77 is required for apoptosis triggering Neoplasia, 16:679-693, DOI: 10.1016/j.neo.2014.08.001 journal’s cover 4,8/10

57 Jangamreddy JR, Panigrahi S, and MJ Łos, (2015) Monitoring of autophagy is complicated - salinomycin as an example BBA Mol. Cell. Res., 1853:604–610. 5,3/2

58 Magnusson K, Appelqvist H, Cieślar-Pobuda A, Wigenius J, Karlsson T, Łos MJ*, Kågedal B, Jonasson J and KPR Nilsson, (2015) Differential vital staining of normal and transformed cells by an anionic conjugated polyelectrolyte Cytometry A, 87:262-72. doi: 10.1002/cyto.a.22627. 3,2/4

59 Moghadam AR, Tutunchi S, Namvaran-Abbas-Abad A, Yazdi M, Bonyadi F, Mohajeri D, Mazani M., Marzban H, Łos MJ and S. Ghavami (2015) Pre-administration of Turmeric Prevents Methotrexate-Induced Liver Toxicity and Oxidative Stress BMC Complement. Altern. Med. 15:246, DOI: 10.1186/s12906-015-0773-6 2,4/2

60 Shakeri R, Hosseinkhani S, Los MJ, Davoodi J, Jain MV, Cieślar Pobuda A, Rafat M, and SK Ardestani (2015) Role of the salt bridge between glutamate 546 and arginine 907 in preservation of autoinhibited form of Apaf-1 Int. J. Biol. Macromol., 81:370-374, doi.org/10.1016/j.ijbiomac.2015.08.027 3,2/1

61 Magnusson KE, Appelqvist H, Cieślar-Pobuda A, Bäck M, Kågedal B, Jonasson JA, Los MJ, and Nilsson KP, An imidazole functionalized pentameric thiophene displays different staining patterns in normal and malignant cells Front Chem, section Chemical Biology, 2015, 3:58. doi: 10.3389/fchem.2015.00058. 0/0

62 Jangamreddy JR, Jain MV, Hallbeck AL, Roberg, K, Lotfi K, and MJ Łos, (2015) Glucose starvation-mediated inhibition of salinomycin induced autophagy amplifies cancer cell specific cell death Oncotarget, 6:10134-45. 5,4/5

63 Jain MV, Jangamreddy JR, Grabarek J, Schweizer F, Klonisch T, Cieslar-Pobuda A, and MJ Łos, (2015) Nuclear localized Akt enhances breast cancer stem like cells through counter-regulation of p21Waf1/Cip1 and p27kip1 Cell Cycle, 14:2109-20. doi: 10.1080/15384101.2015.1041692. 4.2/11

64 Gelmi A, Zhang J, Cieslar-Pobuda A, Ljunngren MK, Los MJ, Rafat M, and EWH Jager (2015) Electroactive 3D materials for cardiac tissue engineering Proc. of SPIE, 9430:94301T-1, doi: 10.1117/12.2084165.

65 Likus W, Siemianowicz K, Markowski J, Wiaderkiewicz J, Kostrząb-Zdebel A, Jura-Szołtys E, Dziubdziela W, Wiaderkiewicz R, and MJ Łos (2016) Bacterial infections and osteoclastogenesis regulators in men and women with cholesteatoma Arch. Immunol. Ther. Exp. 64:241-7. DOI: 10.1007/s00005-015-0373-7. 2,8/0

66 Likus W, Gruszczyńska K, Markowski J, Machnikowska-Sokołowska M, Olczak Z, Bajor G, Baron J, and MJ Los Correlations between structures of nasal cavity in neonates and young infants – CT study Folia Morphologica, 75:334-340, doi: 10.5603/FM.a2015.0128. 0.5/

67 Jain MJ, Shareef A, Likus W, Cieslar-Pobuda A, Ghavami S, and MJ Łos (2016) Inhibition of miR301 enhances Akt-mediated cell proliferation by accumulation of PTEN in the nucleus and effects on cell-cycle regulatory proteins. Oncotarget, 7:20953-20965, DOI: 10.18632/oncotarget.7996. 5,4/0

68 Gelmi A, Cieslar-Pobuda A, de Muinck E, Los M, Rafat M, and E.W.H. Jager (2016) Direct mechanical stimulation of stem cells: a beating electroactive scaffold for cardiac tissue engineering Adv. Health. Mat., 5:1471-80, doi: 10.1002/adhm.201600307. 6/3

69 Cieślar-Pobuda A, Rafat M, Knoflach V, Skonieczna M, Hudecki A, Małecki A, Urasińska E, Ghavami S, and MJ Łos, (2016) Human induced pluripotent stem cell differentiation and direct transdifferentiation into corneal epithelial-like cells Oncotarget, 7:42314-29, DOI: 10.18632/oncotarget.9791. 5,4/

70 Moosavi M, Sharifi M, Ghafary SM, Mohammadalipour Z, Khataee A, Rahmati M, Hajjaran S, Los MJ, Klonisch T, and S Ghavami (2016) Photodynamic N-TiO2 Nanoparticle Treatment Induces Controlled ROS-mediated Autophagy and Terminal Differentiation of Leukemia Cells Sci. Reports, 6:34413, DOI: 10.1038/srep34413. 5.5/

71 Sherrell PC, Cieślar-Pobuda A, Silverå Ejneby M, Sammalisto L, Gelmi A, de Muinck E, Brask J, Łos MJ, and Rafat M. (2017) Rational Design of a Conductive Collagen Heart Patch Macromol. Biosci., (in press), DOI: 10.1002/201600446 3.7

72 Chaabane W, Ghavami S, Małecki A, MJ. Łos (2017) Human-Gyrovirus-Apoptin interferes with the cell cycle and induces G2/M arrest prior to apoptosis Arch. Immunol. Ther. Exp. 65: DOI : 10.1007/s00005-017-0464-8. 2,8/0

73 Hudecki A, Gola J, Ghavami S, Skonieczna M, Markowski J, Likus W, Lewandowska M, Maziarz W, and Marek J. Łos, (2017) Structure and properties of novel tissue bioscaffolds. Med Chem, (in press, DOI: 10.4212/2161-0444.1000427) 1.4/

74 Asl SS, Bergen H, Nikkhah A, Moghadam AR, Ashtari N, Amiri S, Małecki A, Łos MJ, Mehdizadeh M. Pelargonidin exhibits protective effects against amyloid β-induced deficits in the hippocampus of male rats Eur. J. Pharmacol., (under revision, EJP-44484R) 2.7 1. Hudecki A, Skonieczna M, Gola J, Markowski J, Małecki A, Ghavami S, Maziarz W, and Łos MJ, The core-shell PCL/PCL/Ag nanofibers derived by coaxial electrospinning as slow-resorbing biodegradable tissue scaffolds Polymer Degrad. Stabil., (under revision) 1 3.1 2. Puckerta C, Gelmi A, Ljunggren MK, Los MJ, Rafat M, and E.W. H. Jager Optimisation of conductive polymer biomaterials for cardiac progenitor cells RSC Advances, (RA-ART-05-2016-011682 under revision) 3,8 3. Hudecki A, Skonieczna M, Łyko-Morawska D, Gola J, Hawełek Ł, Cieślar-Pobuda A, Adamska J, Mazurek U, Jakóbik-Kolon A, Niedzielska D, Dulski M, Maziarz W, Kolano-Burian A, and Łos MJ Novel, PCL/MWCNT, PCL/MWCNTOH or PCL/MWCNCOOH –based biomaterials for applications in regenerative medicine Eur. J. Pharmacol., (about to be submitted, XXXXXXX) 2.5 4. Cieślar-Pobuda, A., Magnusson, K., Back, M., Nilsson, K.P.R., and M.J. Łos. (2016) Cell stainability with fluorescent thiophene-based ligands JoVE, (under revision, JoVE54222) 1.3 B. Reviews

75 Schulze-Osthoff, K. and M. Los, (1995) Excitements about cell death. Diabetes Prevention & Therapy 9: 22-23.

76 Schulze-Osthoff, K., Los, M. and P.A. Baeuerle, (1995). Redox signalling by transcription factors NF-kB and AP-1 in lymphocytes. Biochem. Pharmacol. 50: 735-741. 4,8/218

77 Schulze-Osthoff, K., Bauer, M., Vogt, M. and M. Los, (1996) Role of ICE-related and other proteases in Fas-mediated apoptosis. Cell Death Diff. 3: 177-84. 8/21

78 Debatin, K.M., Beltinger, C., Bohler, T., Fellenberg, J., Friesen, C., Fulda, S., Herr, I., Los, M., Scheuerpflug, C., Sieverts, H. and K. Stahnke, (1997) Regulation of apoptosis through CD95 (APO-I/Fas) receptor-ligand interaction. Biochem Soc. Trans. 25: 405-410. 2,8/23

79 Schulze-Osthoff, K., Ferrari, D., Los, M., Wesselborg, S. and M.E. Peter, (1998) Apoptosis signaling by death receptors. Eur. J. Biochem. 254: 439-59. (curr. exists as FEBS Journal) 4,1/708

80 Los, M., Wesselborg, S., and K. Schulze-Osthoff (1999) The role of caspases in development, immunity and apoptotic signal transduction: lessons from knock-out studies. Immunity 10: 629-39. 22,1/306

81 Los, M., Stroh, C., Jänicke, R.U., Engels, I.H. and K. Schulze-Osthoff, (2001) Caspases: more than just killers? Trends Immunol. 22: 31-4. 10,5/136

82 Michalke, M., Stroh, K., Chlichlia, K., Stepczynska, A., Mozoluk, M., and M. Los, (2001) The emerging role of caspases in signal transduction as revealed by knock-out studies - not only apoptosis. Signal Transd. 2:51-65, doi: 10.1002/1615-4061(200111)1:1/2<51::AID-SITA51>3.0.CO;2-F

83 Sadowski-Debbing, K., Coy, J.F., Mier, W., Hug, H. and M. Los, (2002) Caspases – their role in apoptosis and other physiological processes as revealed by knock-out studies. Arch. Immunol. Ther. Exp. 50:19-34. 2,8/32

84 Chlichlia, K., Los, M., Schulze-Osthoff, K., Gazzolo, L., Schirrmacher, V. and K. Khazaie, (2002) Redox Events in HTLV-1 Tax-induced Apoptotic T-cell Death. Antioxid. Redox. Signal. 4:471-7. 7,2/13

85 Los, M., Burek, C,J., Stroh, C., Benedyk, K., Hug, H. and A. Mackiewicz (2003) Anticancer drugs of Tomorrow - Apoptotic Pathways as Target for Drug Design. Drug Discov. Today 8:67-77. 6,1/80

86 Cassens, U., Lewinski, G., Samraj, A.K., von Bernuth, H., Baust, H., Khazaie K. and M. Los, (2003) Viral modulation of cell death by inhibition of caspases. Arch. Immunol. Ther. Exp. 51:19-27. 2,8/21

87 Jurisic, V., Renz, A. and M. Los, (2003) Estimation of cell membrane alteration after drug treatment by LDH release. Blood 101:2894-5. 10,2/0

88 Johar, D., Roth, J.C., Bay, G.H., Walker, J.N., Kroczak, T.J., Los, M. (2004) Inflammatory response, reactive oxygen species, programmed (necrotic-like and apoptotic) cell death and cancer. Roczniki Akademii Medycznej w Białymstoku, 49:31-9.

89 Kreuter, M., Langer, C., Kerkhoff, C., Reddanna, P., Kania, A.L., Maddika, S., Chlichlia, K., Bui, N.T. and M. Los, (2004) Stroke, myocardial infarct, acute and chronic inflammatory diseases: Caspases and other apoptotic molecules as targets for drug development. Arch. Immunol. Ther. Exp. 52:141-55. 2,8/31

90 Philchenkov, A., Zavelevich, M., Kroczak, T. J. and M. Los (2004) Caspases and cancer: mechanisms of inactivation and new treatment modalities. Exp. Oncology 26:82-97. /92

91 Mendoza, F.J., Espino, P., Cann, K.L., Bristow, N., McCrea, K. and M. Los (2005) Anti-tumor chemotherapy utilizing peptide-based approaches – apoptotic pathways, kinases and proteasome as targets. Arch. Immunol. Ther. Exp. 53:47-60. 2,8/49

92 Booy, E.P., Kadkhoda, K., Johar, D., Bay, G.H., and M. Los (2005) The immune system, involvement in neurodegenerative diseases, ageing and cancer. Curr. Med. Chem.-Anti-Inflammatory & Anti-Allergy Agents 4:349-53. 0/

93 Ghavami, S., Hashemi, M., Kadkhoda, K., Alavian, S. M., Bay, G. H. and M. Los. (2005) Apoptosis in Liver Diseases – Detection and Therapeutic Applications. Med. Sci. Monitor 11:RA337-345. 1,3/62

94 Hauff K, Zamzow C, Law WJ, De Melo J, Kennedy K. and M. Los (2005) Peptide-based approaches to treat asthma, arthritis, other autoimmune diseases and pathologies of the central nervous system. Arch Immunol Ther Exp. 53:308-20. 2,8/21

95 Krzemieniecki, K., Szpyt, E., Rashedi, I, Gawron, K, Los, M. (2006) Targeting of solid tumors and blood malignancies by antibody-based therapies - EGFR-pathway as an example Centr. Eur. J. Biol. (Open Life Sci.), 1:167-82, DOI: 10.2478/s11535-006-0014-6 0,9/15

96 Kroczak, T.J., Baran, J., Pryjma, J., Siedlar, M., Reshedi, I., Hernandez, E., Alberti, E., Maddika, S., Los, M. (2006) The emerging importance of DNA mapping and other comprehensive screening techniques, as tools to identify new drug targets and as a means of (cancer) therapy personalisation Expert Opinion on Therapeutic Targets, 10:289-302. 4,6/9

97 Booy, E.P., Johar, D., Maddika, S., Pirzada, H., Sahib, M.M., Gehrke, I., Loewen, S., Louis, S.F., Kadkhoda, K., Mowat, M., and M. Los (2006) Monoclonal and Bispecific Antibodies as Novel Therapeutics. Arch. Immunol. Ther. Exp. 54:85-101. 2,8/15

98 Anderson, J.E., Hansen, L.L., Mooren, F.C., Post, M., Hug, H., Zuse, A., and M. Los. (2006) Methods and biomarkers for the diagnosis and prognosis of cancer and other diseases: Towards personalized medicine. Drug Resist. Updat. 9:198–210. 10,5/31

99 Maddika, S., Mendoza, F.J., Hauff, K., Zamzow, C.R., Paranjothy, T., and M. Los (2006) Cancer-selective therapy of the future – apoptin and its mechanism of action. Cancer Biol. Ther. 5:10-19. 3,4/61

100 Banerji, S. and M. Los (2006) Important Differences between Topoisomerase-I and -II Targeting Agents. Cancer Biol. Ther. 5:965-966. 3,4/8

101 Johnston, J.B., Navaratnam, S., Pitz, M.W., Maniate, J.M., Wiechec, E., Baust, H., Gingerich, J., Skliris, G.P., Murphy, L.C. and M. Los. (2006) Targeting the EGFR pathway for cancer therapy. Curr. Med. Chem. 13:3483-92. 3,8/104

102 Maddika, S., Ande, S.R., Panigrahi, S., Paranjothy, T., Weglarczyk, K., Zuse, A., Eshraghi, M., Manda, K.D., Wiechec, E. and M. Los (2007) Cell survival, cell death and cell cycle pathways are interconnected: implications for cancer therapy. Drug Resist Updat. 10:13-29. (since publication, continuously among top 10 dowloaded papers from journal’s web page), designated, by Web of Science as HIGHLY CITED -> ranked within top 1% of the field, 10,5/247

103 Henson, E.S., Johnston, J.B., Los M. and S.B. Gibson (2007) Clinical outcomes of epidermal growth factor receptor family inhibitors in breast cancer. Biologics: Targets and Therapies 1:229-39.

104 Alexander, H.K., Booy, E.P., Xiao, W., Ezzati, P., Baust, H., Los, M. (2007) Selected technologies to control genes and their products for experimental and clinical purposes Arch. Immunol. Ther. Exp. 55:139-149. 2,8/16

105 Rashedi, I., Panigrahi, S., Ezzati P., Ghavami, S. and M. Los, (2007) Autoimmunity and Apoptosis – therapeutic implications. Curr. Med. Chem. 14:3139-59. 3,8/25

106 Hombach-Klonisch, S., Paranjothy, T., Wiechec, E., Pocar, P., Mustafa, T., Seifert, A., Zahl, C., Gerlach, K.L., Biermann, K., Steger, K., Hoang-Vu, C., Schulze-Osthoff, K. and M. Los. (2008) Cancer Stem Cells as Targets for Cancer Therapy – Selected Cancers as Examples. Arch. Immunol. Ther. Exp. 56:165-80. 2,8/36

107 Panigrahi, S., Klonisch, T. and M. Los. (2008) The art of killing: double stroke with apoptin and survivin as a novel approach in cancer therapy. Cancer Biol. Ther. 7:1061-2. 3,4/6

108 Klonisch, T., Wiechec, E., Hombach-Klonisch, S., Ande, S.R., Wesselborg, S., Schulze-Osthoff, K. and M. Los. (2008) Cancer stem cell markers in common cancers – therapeutic implications. Trends Mol. Med. 14:450-460. 10,1/144

109 Hombach-Klonisch, S., Panigrahi, S., Rashedi, I., Seifert, A., Alberti, E., Pocar, P., Kurpisz, M., Klonisch, T., Schulze-Osthoff, K., Mackiewicz, A. and M. Los. (2008) Adult stem cells and their trans-differentiation potential - perspectives and therapeutic applications. J. Mol. Med. 86:1301-14. 4,5/68

110 Gurevich-Panigrahi , T., Panigrahi, S. Wiechec, E. and M. Los. (2009) Obesity: Pathophysiology and Clinical Management. Curr. Med. Chem. 16:506-21. 3,8/34

111 Los, M., Maddika, S., Erb, B. and K. Schulze-Osthoff (2009) Switching Akt's survival signaling to deadly responses BioEssays 31:492-5. 4,8/55

112 Los, M., Panigrahi, S., Rashedi, I., Mandal, S., Stetefeld, J., Essmann, F. and K. Schulze-Osthoff (2009) Apoptin, a tumor-selective killer. BBA Mol. Cell. Res., 1793:1335-1342. 5,3/49

113 Los, M. and K. Schulze-Osthoff (2009) Catching chromatin relaxation in act by flow cytometry Cell Cycle, 8:2140-1. 4,2/0

114 Marek Los, 2009, New, exciting developments in experimental therapies in the early 21st century. Eur. J. Pharm., 625:1-5. 2,7/11

115 Ghavami, S., Hashemi, M., Ande, S.R., Yeganeh, B., Xiao, W., Eshraghi, M., Bus, C., Kadkhoda, K., Wiechec, E., Halayko, A.J. and M. Los, (2009) Apoptosis and Cancer: Mutations within Caspase Genes J. Med. Genet., 46:497–510. (“editor’s choice”, among the “10 Most-Frequently Read Articles“ since September-09 – until January-13), designated, by Web of Science as HIGHLY CITED -> ranked within top 1% of the field 5,7/245

116 Vincent, F.C. and M. Los, (2011) New potential instrument to fight hepatocellular cancer by restoring p53. Hepat. Mon. 11:331-2. 1,8/4

117 Alavian, S.M., Ande, S.R., Coombs, K., Davoodpour, P., Hashemi, M., Los, M., and S. Ghavami, (2011), Virus-Triggered Autophagy in Viral Hepatitis – possible novel strategies for drug development. J. Viral Hepatitis 18:821-30. 3,6/20

118 Jangamreddy J.R. and M.J. Los, (2012) Mitoptosis, a novel mitochondrial death mechanism leading predominantly to activation of autophagy Hepat. Mon. 12:49-51. 1,8/14

119 Chaabane, W., User, S.D., El-Gazzah, M., Jaksik, R., Sajjadi, E., Rzeszowska-Wolny, J. and M. Łos, (2013), Autophagy, Apoptosis, Mitoptosis and Necrosis - Interdependence between those Pathways and Effects on Cancer Arch. Immunol. Ther. Exp. 61:43-5. (doi: 10.1007/s00005-012-0205-y). (journal’s most popular article“ in 2013, and 2014, cited already over 50 times.) 2,8/72

120 Jain, M.V., Paczulla, A.M., Dimgba, F.N., Klonisch, T., Rao, S.B., Roberg, K., Schweizer, F., Lengerke, C., Davoodpour, P. and M. Łos, (2013), Interconnections between Apoptotic, Autophagic and Necrotic pathways: Implications for Cancer Therapy Development. J. Cell. Mol. Med., 17:12-29, doi: 10.1111/jcmm.12001. 4,6/80

121 Yeganeh, B., Hashemi, M., de Serres, F.J., Los, M.J., and S. Ghavami, (2013) Different Faces of Hepatocellular Carcinoma as a Health Threat in 21st Century Hepat. Mon. 2013;13:e9308. DOI: 10.5812/hepatmon.9308 1,8/3

122 Cieślar-Pobuda, A and M.J. Łos, (2013), Prospects and limitations of “click-chemistry”-based DNA labeling technique employing 5-ethynyl-2’deoxyuridine (EdU). Cytometry A, 83:977-8. (DOI: 10.1002/cyto.a.22394) 3,2/5

123 Jain, M.V., A and M.J. Łos, (2013), Spatiotemporal Cytometry—Simultaneous Analysis of DNA Replication and Damage. Cytometry A, 83:975-6. (DOI: 10.1002/cyto.a.22399) 3,2/1

124 Ghavami, S., Shojaei, S., Ande, S.R., Jangamreddy, J.R., Yeganeh, B., Mehrpour, M., Christoffersson, J., Chaabane, W., Moghadam, A.R., Hashemi, M. Owji, A.A., and M.J. Łos, (2014), Autophagy and Apoptosis Dysfunction in Neurodegenerative Disorders; autophagy and apoptosis-directed experimental treatments Progress Neurobiol., 112:24-49, doi: 10.1016/j.pneurobio.2013.10.004 (journal’s most frequently downloaded article“ in 2014 and in 2015, over 11 000 downloads just unlit Sept.-14;also editors choice), designated, by Web of Science as HIGHLY CITED -> ranked within top 1% of the field, cited over 140 times as for August-16 11,2/141

125 Wasik, A.M., Grabarek, J., Pantovic, A., Cieślar-Pobuda, A., Asgari, H.R., Bundgaard-Nielsen, C., Rafat, M., Ghavami, S., and M.J. Łos, (2014) Reprogramming and carcinogenesis - parallels and distinctions Int. Rev. Cell Mol. Biol. 308:167-203. doi: 10.1016/B978-0-12-800097-7.00005-1. 4,1/14

126 Savelyeva A, Ghavami S, Davoodpour P, Asoodeh A, Los MJ. An overview of brevinin superfamily: structure, function and clinical perspectives. Adv Exp Med Biol. 2014;818:197-212. doi: 10.1007/978-1-4471-6458-6_10. 1,8/1

127 Rollano Peñaloza OM, Lewandowska M, Stetefeld J, Ossysek K, Madej M, Bereta J, Sobczak M, Shojaei S, Ghavami S, and MJ Łos, (2014), Apoptins: selective anticancer agents Trends Mol. Med. 20:519-528, doi: 10.1016/j.molmed.2014.07.003. 10,1/11

128 Likus W, Siemianowicz K, Bieńk K, Pakuła M, Pathak H, Dutta C, Wang Q, Assaraf YG, Ghavami S, Cieślar-Pobuda A, and MJ Łos (2016) Could drugs inhibiting the mevalonate pathway also target cancer stem cells? Drug Resist. Updat., 25:13–25, doi: 10.1016/j.drup.2016.02.001 10,5/3

129 Klionsky DJ, Abdelmohsen K, Abe A, Abedin MJ, ... Los M, et al., (2016), Guidelines for the Use and Interpretation of Assays for Monitoring Autophagy (3rd edition) Autophagy, 12:1-222. 12/175

130 Akbari-Birgani S, Paranjothy T, Zuse A, Janikowski T, Cieślar-Pobuda A, Likus W, Urasińska E, Schweizer F, Ghavami S, Klonisch T, and MJ Łos, (2016) Cancer stem cells/cancer-initiating cells and methods of their detection Drug Discov. Today, S1359-6446(16)30073-3, doi: 10.1016/j.drudis.2016.03.004. 6,1/3

131 Mokarram P, Albokashy MA, Zarghooni M, Mousavi MA, Sepehri Z, Chen QM, Hudecki A, Sargaz A, Alizadeh J, Moghadam AR, Hashem M, Klonisch T, Owji AA, Los MJ, and S. Ghavami (2017) New Frontier in the Treatment of Colorectal Cancer: Autophagy and Unfolded Protein Response as Targets Autophagy, 23:1-39, doi: 10.1080/15548627.2017.1290751. 12/

132 Knoflach V, Cieślar-Pobuda A, Ringh MV, Stark J, Likus W, Siemianowicz K, Ghavami S, Hudecki A, Green JL, and MJ Łos, Trans-differentiation and reprogramming; overview of the processes, their similarities and differences BBA Mol. Cell. Res., DOI:10.1016/j.bbamcr.2017.04.017 5,3/ Citation Graph, Marek Los C.

Books

1. Caspases - their role in cell death and cell survival. edited by M. Los & H. Walczak; Kluwer Academic Press (2002), ISBN: 1-58706-132-5.

2. Los, M. (2005) (volume edition) Immunity and Cancer. Curr. Med. Chem.-Anti-Inflammatory & Anti-Allergy Agents, 4:349-448.

3. “Apoptotic pathways as target for novel therapies of cancer and other diseases.” edited by M. Los & S.B. Gibson; Springer Science+Business Media, Inc. (2005), ISBN: 0-387-23384-9.

4. Marek Los, (2009), (special vol. edition) “New vistas in Anti-Cancer Therapy“ Eur. J. Pharm., vol. 625. 2,5

5. Ghavami, S., Los, M.J., de Serras, F.J. (2012), (special issue edition) “Hepatocellular Carcinoma” Hepat. Mon. vol.12(10) (special issue edition, dedicated Hepatocellular carcinoma) 1,9 D. Book chapters

6. Los, M., Walczak, H., Schulze-Osthoff, K. and J.C. Reed (2000) Fluorogenic substrates as detectors of caspase activity during Natural Killer Cell- induced apoptosis. in: Natural Killer Cell Protocols, eds. K. S. Campbell & M. Colonna; Methods in Molecular Biology; Humana Press ISBN: 0-89603-683-9.

7. Michalke, M., Stepczynska, A., Burek, M., Loser, K., Krzemieniecki, K. and M. Los. Caspases as targets for drug development. in: Caspases - their role in cell death and cell survival, edited by M. Los & H. Walczak; Kluwer Academic Press (2002), ISBN: 1-58706-132-5.

8. Ghavami, S., Barczyk, K., Maddika, S., Vogl, T., Steinmüller, L., Pour-Jafari, H., Evans, J.A., and M. Los. Monitoring of programmed cell death in vivo and in vitro, –new and old methods of cancer therapy assessment. in: “Apoptotic pathways as target for novel therapies in cancer and other diseases.” edited by M. Los & S.B Gibson; Springer Science+Business Media, Inc. (2005), ISBN: 0-387-23384-9.

9. Ghavami, S., Ande, S.R., Hashemi, M., Chitayat, S., Chazin, W.J., Halayko, A.J., Karami-Tehrani, F., Kerkhoff, C. and M. Los Apoptin, Brevinin-2R, and S100A8/A9; three anticancer proteins and peptides with different mechanisms of action. In “Proteins Killing Tumour Cells”, Series: Cell Death and Disease Eds. Backendorf, Borst, Noteborn, Tavassoli, Research-Signpost (2009), ISBN: 978-81-7895-405-9.

10. Los, M., Rashedi, I., Panigrahi, S., Klonisch, T. and K. Schulze-Osthoff, “Tumor Growth and Cell Proliferation”, in: Medical Radiology: volume: The Impact of Tumor Biology on Cancer Treatment and Multidisciplinary Strategies, Eds.: Molls, M., Vaupel, P., Nieder, C., Anscher, M.S., (2009), Springer Science+Business Media, Inc. ISBN 978-3-540-74385-9.

11. Savelieva, A., Ghavami, S., Davoodpour, P., Asoodeh, A., and M. J. Łos, “An Overview of Brevinin Superfamily: Structure, Function and Clinical Perspectives“, in “Anticancer Genes”, Ed.: Grimm, S., (2014), Springer Science+Business Media, Inc. ISBN 978-1-4471-6457-9 ISBN 978-1-4471-6458-6 (eBook), DOI 10.1007/978-1-4471-6458-6.