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Laboratory of Monoclonal Antibodies

Group Leader

 

 

Dr Monika Bzowska

tel. 48 12 664 64 05

email: monika.bzowska@uj.edu.pl 

 

Team members

Dr Monika Bzowska tel. 48 12 664 64 05 monika.bzowska@uj.edu.pl
Mgr Tomasz Klaus tel. 48 12 664 64 05 tomasz.klaus@doctoral.uj.edu.pl, Asystent Naukowy

Summary

Generation of novel biomolecules based on monoclonal antibodies is in the laboratory’s focus. The classical hybridoma technology is useful for production of full-size monoclonal antibodies that may serve as valuable tools for research, diagnostics or therapy. An alternative technology based on phage-displayed antibody fragments is also available to address a broader scope of target molecules. Both methods are used to obtain proteins that are exclusively specific and bind the antigen with high affinity. Additional expertise of the research team is in antibody modifications that improve their research, diagnostic or therapeutic potential. The laboratory has the capacity to produce monoclonal antibodies using a disposable bag bioreactor (BIOSTAT CultiBag RM by Sartorius Stedim) and hybridoma cell cultures of up to 25 litre volume. Given the high antibody concentrations obtainable in the bag culture, the production yield can be as high as 1 gram per litre.
The R&D staff offers scientific collaboration and contract research services in the area of monoclonal antibodies, including generation, purification and modification of antibodies of interest, as well as scaling-up of the production process from micrograms to grams of protein.

Grants

  1. NCN Preludium (Tomasz Klaus) 2015-2018

Selected publications

  1. Klaus T, Bzowska M, Kulesza M, Kabat AM, Jemioła-Rzemińska M, Czaplicki D, Makuch K, Jucha J, Karabasz A, Bereta J. Agglutinating mouse IgG3 compares favourably with IgMs in typing of the blood group B antigen: Functionality and stability studies. Sci Rep. 2016 Aug 3;6:30938. doi: 10.1038/srep30938.
  2. Ossysek K, Uchański T, Kulesza M, Bzowska M, Klaus T, Woś K, Madej M, Bereta J. A new expression vector facilitating production and functional analysis of scFv antibody fragments selected from Tomlinson I+J phagemid libraries. Immunol Lett. 2015 Oct;167(2):95-102.
  3. Wladyka B, Piejko M, Bzowska M, Pieta P, Krzysik M, Mazurek Ł, Guevara-Lora I, Bukowski M, Sabat AJ, Friedrich AW, Bonar E, Międzobrodzki J, Dubin A, Mak P. A peptide factor secreted by Staphylococcus pseudintermedius exhibits properties of both bacteriocins and virulence factors. Sci Rep. 2015 Sep 28;5:14569.
  4. Klaus T, Kulesza M, Bzowska M, Wyroba B, Kilarski WW, Bereta J. Overcoming inefficient secretion of recombinant VEGF-C in baculovirus expression vector system by simple purification of the protein from cell lysate. Protein Expr Purif. 2015 Jun;110:151-8.