Łabuz Justyna, dr, Group Leader, firstname.lastname@example.org, (+48 12) 664 6109
Banaś Agnieszka Katarzyna, dr, email@example.com, (+48 12) 664 6410
Bażant Aneta, mgr, firstname.lastname@example.org, (+48 12) 664 6109
Hermanowicz Paweł, mgr, email@example.com, (+48 12) 664 6347
Kozłowska Anna, mgr inż., firstname.lastname@example.org, (+48 12) 664 6109
Zgłobicki Piotr, mgr, email@example.com, (+48 12) 664 6109
The project currently carried out in MCB focuses on the mechanisms through which light controls phototropin expression in Arabidopsis thaliana. We study transcription factors that bind to phototropin promoters and investigate the involvement of post-transcriptional mechanisms in the regulation of phototropin expression.
The second topic investigated by our group is regulation of plant functioning by UVA/blue light and the properties of plant photolyases. Photolyases are enzymes involved in the direct repair of UVB-induced pyrimidine dimers in a blue light/UVA-dependent manner. This reaction is called photoreactivation. We study the subcellular localization of both well-known and putative Arabidopsis photolyases using proteins fused to fluorescent proteins. We also analyze the effects of visible and UV light on photolyase expression and activity.
We plan to:
- investigate the molecular basis of phototropin signaling leading to chloroplast movements, in particular the physiological relevance of phototropin dimerization.
- elucidate the biological activity of well-known and putative Arabidopsis photolyases in terms of photoreactivation, chloroplast biogenesis, chloroplast DNA maintenance and plant responses to abiotic stresses. The impact of alternative splicing, sumoylation and phosphorylation on biological functions of these genes will be investigated.
- start a cooperation with a commercial partner to develop photocatalytically active anti-foaming surfaces resistant to algae and moss growth.
2015 – 2018 – SONATA8, NCN, UMO-2014/15/D/NZ2/02306 “Light regulation of phototropin expression in Arabidopsis thaliana”, principle investigator: Justyna Łabuz
2014 - 2019 – SONATA BIS3 UMO-2013/10/E/NZ1/00749 "Characterizing the functions of plant PCNA1 and PCNA2 proteins using Arabidopsis thaliana as an experimental model", principle investigator: Wojciech Strzałka
2017 – 2022 – SONATA BIS6, NCN, UMO-2016/22/E/NZ3/00326, „ Arabidopsis photolyases: the role of post-transcriptional and post-translational modifications, influence on DNA repair, chloroplast functioning and plant responses to abiotic stresses.” principle investigator: Agnieszka Katarzyna Banaś
2018 – 2021 OPUS 13, Dissecting the molecular basis of phototropin signaling to chloroplast movements in Arabidopsis thaliana, principle investigator: Justyna Łabuz
Sztatelman O, Łabuz J, Hermanowicz P, Banaś AK, Bażant A, Zgłobicki P, Aggarwal C, Nadzieja M, Krzeszowiec W, Strzałka W, Gabryś H 2016. Fine tuning chloroplast movements through physical interactions between phototropins. Journal of Experimental Botany, 67, 4963-4978.
Łabuz J, Samardakiewicz S, Hermanowicz P, Wyroba E, Pilarska M, Gabryś H 2016. Blue light-dependent changes in loosely bound calcium in Arabidopsis mesophyll cells: an X-ray microanalysis study. Journal of Experimental Botany, 67, 3953-3964.
Eckstein A, Jagiełło-Flasińska D, Lewandowska A, Hermanowicz P, Appenroth K-J, Gabryś H 2016. Mobilization of storage materials during light-induced germination of tomato (Solanum lycopersicum) seeds. Plant Physiology and Biochemistry, 105, 271-281.
Sztatelman O, Grzyb J, Gabryś H, Banaś AK 2015. The effect of UV-B on Arabidopsis leaves depends on light conditions after treatment. BMC Plant Biology 15, 281.
Strzałka W, Aggarwal C, Krzeszowiec W, Jakubowska A, Sztatelman O, Banaś AK 2015. Arabidopsis PCNAs form complexes with selected D-type cyclins. Frontiers in Plant Science 6, 516.
Grzyb J, Gieczewska K, Łabuz J, Sztatelman O. 2018. Detailed characterization of Synechocystis PCC 6803 ferredoxin:NADP+ oxidoreductase interaction with model membranes. Biochimica et Biophysica Acta (BBA) - Biomembranes. 1860, 281-291
Kowalska E, Bartnicki B, Fujisawa R, Bonarek P, Hermanowicz P, Tsurimoto T, Muszyńska K, Strzalka W. 2018. Inhibition of DNA replication by an anti-PCNA aptamer/PCNA complex. Nucleic Acids Research, gkx1184, https://doi.org/10.1093/nar/gkx1184
Banaś AK, Hermanowicz P, Sztatelman O, Łabuz J, Aggarwal Ch, Zgłobicki P, Jagiełło-Flasińska D, Strzałka W 2018. 6,4 - PP Photolyase Encoded by AtUVR3 is Localized in Nuclei, Chloroplasts and Mitochondria and Its Expression is Down-Regulated by Light in a Photosynthesis-Dependent Manner. Plant and Cell Physiology. pcx159, https://doi.org/10.1093/pcp/pcx159