Plant-Microorganism Interactions Group

Group leader

 

  Dr Piotr Rozpądek

  T: +4812 664 6108

  E: piotr.rozpadek@uj.edu.pl

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Team members

  • Dr Piotr Rozpądek - lider grupy
    piotr.rozpadek@uj.edu.pl
    T: 12664 6108
  • Dr inż. Rafał Ważny
    rafal.ważny@uj.edu.pl
    T: 12664 6108
  • Mgr Martyna Janicka
    martyna.janicka@uj.edu.pl
    T: 12664 6118
  • Mgr Marta Śliwa 
    martajolanta.sliwa@student.uj.edu.pl
    T: 12664 6118
  • Mgr Weronika Janas
    weronika.janas@uj.edu.pl
    T: 12664 6118
  • Mgr Agnieszka Domka
    agnieszka.domka@doctoral.uj.edu.pl
    T: 12664 6118
  • Dr inż. Roman Jędrzejczyk
    roman.jedrzejczyk@uj.edu.pl
    T: 12664 6118

Summary

The activity of the Plant-Microorganism Interactions Group of the MCB dates back to 2014. Our investigations focus on the mechanisms of mutualistic plant-microorganism interactions and the utilization of symbiotic microbes in improving the efficiency of phytoremediation and in sustainable agriculture. Our scientific activities are carried out in tight collaboration with prof. Katarzyna Turnau’s “Plant-Microbe Interactions Group” from the Institute of Environmental Science JU. 

Currently we are working on 3 projects funded by NCBiR and NCN:
 
1.    We are testing the possibility of using fungal endophytes as plant supporting agents in phytoremediation of environments highly polluted with toxic metals in the “Endophytes as a novel tool in phytoremediation” NCN MAESTRO project. Our investigations focus around issues concerning:
a.    The mechanisms of fungi dependent root hair elongation, the interaction between ethylene, auxin and strigolacotone in root hair elongation,
b.    Symbiosis induced alterations in metal and nutrient homeostasis and distribution,
c.    The role of strigolactone in the parasite-endophyte continuum.
 
Rys. Schemat ilustrujący metodę opracowania  konsorcjów stosowanych w bioremediacji i rolnictwie. Układy te charakteryzują się zwiększoną tolerancją na działanie niekorzystnych czynników środowiskowych
 
Fig. Scheme illustrating the development of plant-microbe consortia with enhanced stress tolerance potential for bioremediation and agriculture
 
 
Fig. GFP-labelled (green fluorescent protein) endophytic fungi Mucor sp. in root epidermal cells of Arabidopsis arenosa
 

2.    In collaboration with the „Niva“ berry fruit plant seedling production company we are developing a microorganism based biotisation technology which will allow to reduce the use of inorganic fertlizers and chemical pest control agents (BERRY Development of biotisation techniques for comercial production of organic berry fruits). As a part of this project we are also investigating functioanl and structural changes in the photsynthesis aparatus of biotisized plants.

 

Fig. (A) blueberry seedling, (B) chlorophyll a fluorescence of Arabidopsis thaliana

3.    In the ERA-NET (NCBR): “Developing Ni agromining on ultramafic land in Europe” fungal endophytes are tested for their ability to improve Ni accumulation in shoots of Ni hyperacumulator plant species, used in Ni phytomining. The project is carried out in collaboration with 7 other institutions, both academic and from the industry, European leaders in Ni phytomining. The research conducted in our lab focuses on:
a.    Improving Ni accumulation in plant shoots by inoculation with specific fungal endophytes,
b.    Identification of fungi species capable to improve bioremediation techniques,
c.    Identification of heritable and inheritable mechanisms in Ni hyperaccumulation. 
Additionally, several services for the industry have been provided. The most important was developing and evaluating mycorrhiza inoculum for crop production, the evaluation of microbiological contamination of artworks and developing artwork preservation strategies and molecular identification of ectomycorrhizal fungi used for the production of commercial inoculum.
 

Projects

a) MAESTRO (NCN): Endofity jako nowe narzędzie fitoremediacji,
b) TANGO (NCBR): Opracowanie technologii biotyzacji do komercyjnej, ekologicznej produkcji owoców jagodowych,
c) ERA-NET (NCBR): Developing Ni agromining on ultramafic land in Europe,
d) OPUS (NCN): Czy remediacja kadmu zależy od typu metabolizmu fotosyntetycznego?

Publications

  1. Jodłowski P.J., Jędrzejczyk R.J., Chlebda D, Gierada M., Łojewska J. 2017; In situ spectroscopic studies of methane catalytic combustion over Co, Ce, and Pd mixed oxides deposited on a steel surface; Journal of Catalysis, 350:1–12
  2. Jodłowski P., Jędrzejczyk R.; Garncarczyk A.; Łojewska J.; Kołodziej A. 2017. New method of determination of intrinsic kinetic and mass transport parameters from typical catalyst activity tests: problem of mass transfer resistance and diffusional limitation of reaction rate; Chemical Engineering Science, 162:322–331
  3. Ważny R., Kowalski S. 2017. Ectomycorrhizal fungal communities of silver-fir seedlings regenerating in fir stands and larch forecrops. Trees, DOI 10.1007/s00468-016-1518-y
  4. Rozpadek, P., Wezowicz, K., Stojakowska, A., Malarz, J., Surówka, E., Sobczyk, T., Anielska, T., Wazny, R., Miszalski, Z., Turnau, K. 2014. Mycorrhizal fungi modulate phytochemical production and antioxidant activity of Cichorium intybus L. (Asteraceae) under metal toxicity. Chemosphere, 112:217-224.
  5. Rozpadek P., Wezowicz K., Nosek M., Wazny R., Tokarz K., Lembicz M., Miszalski Z., Turnau K. 2015. The fungal endophyte Epichloe typhina improves photosynthesis efficiency of its host orchard grass (Dactylis glomerata). Planta, 242/ 4:1025-1035. 
  6. Wężowicz K., Turnau K., Anielska T., Zhebrak I., Gołuszka K., Błaszkowski J., Rozpadek P. 2015. Metal toxicity differently affects the Iris pseudacorus-arbuscular mycorrhiza fungi symbiosis in terrestrial and semi-aquatic habitats. Environmental Science and Pollution Research. 1-8.
  7. Rozpadek P., Nosek M., Slesak I., Kunicki E., Dziurka M., Miszalski Z. 2015, Ozone fumigation increases the abundace of nutrients in Brassica vegetables: broccoli (Brassica oleracea var.italica) and Chinese cabbage (Brassica pekinensis), European Food Research Technol. 240, 459-462
  8. Libik-Konieczny M., Kozieradzka-Kiszkurno M., Desel Ch., Michalec-Warzecha Ż. Miszalski Z., Konieczny R. 2015. The localization of NADPH-oxidase and reactive oxygen species in in vitro-cultured Mesembryanthemum crystallinum L. hypocotyls discloses their differing roles in rhizogenesis, Protoplasma 252, 477-487
  9. Kocurek, M., Kornas, A., Pilarski J., Tokarz K., Lüttge U., Miszalski Z. 2015. Photosynthetic activity of stems in two Clusia species. Trees 29,1029-1040
  10. Nosek M., Rozpadek P., Kornas A., Kuzniak E., Miszalski Z. 2015, Veinal-mesophyll interaction under biotic stress, J. Plant Physiol. 185:52-56
  11. Nosek M., Kornas A., Kuzniak E., Miszalski Z. 2015. Plastoquinone pool redox state modifies plant response to pathogen, Plant Physiol. Biochem. 96, 163-170 
  12. Kwiatkowska, M., Ważny, R., Turnau, K., Wójcik, A. 2016. Fungi as deterioration agents of historic glass plate negatives of Brandys family collection. International Biodeterioration and Biodegradation. 115: 133-140.
  13. Rozpadek, P., Rapała-Kozik, M., Wezowicz, K., Grandin, A., Karlsson, S., Wazny, R. Anielska, T., Turnau, K. 2016. Arbuscular mycorrhiza improves yield and nutritional properties of onion (Allium cepa). Plant Physiology and Biochemistry. 107: 264-272. 
  14. Kryca J., Iwaniszyn M., Piątek M., Jodłowski P. J., Jędrzejczyk R., Pędrys R., Wróbel A., Łojewska J., Kołodziej A., 2016. Structured Foam Reactor with CuSSZ-13 Catalyst for SCR of NOx with Ammonia, Top. Catal.
  15. Jodłowski P. J., Jędrzejczyk R. J., Chlebda D., Tyczkowski J., Kryca J., Kołodziej A., and Łojewska J., 2016. Structure effects on activity of plasma deposited cobalt oxide catalysts for VOC combustion, Top. Catal., pp. 1-8,
  16. Jodłowski P. J., Chlebda D., Piwowarczyk E., Chrzan M., Jędrzejczyk R. J., Sitarz M., Węgrzynowicz A., Kołodziej A., Łojewska J., 2016. In situ and operando spectroscopic studies of sonically aided catalysts for biogas exhaust abatement,” J. Mol. Struct., Volume 1126, Pages 132–140,
  17. Ważny R. 2014. Ectomycorrhizal communities associated with silver fir seedlings (Abies alba Mill.) differ largely in mature silver fir stands and in Scots pine forecrops. Annals of Forest Science, 71(7):801-810
  18. Pajdak-Stós A., Ważny R., Fiałkowska E. 2016. Can a predatory fungus (Zoophagus sp.) endanger the rotifer populations in activated sludge? Fungal Ecology 23:75-78 
  19. Halama M., Ważny R., Czosnykowska-Łukacka M., Dobosz T. 2016. Tricholoma ustaloides (Agaricales, Basidiomycota) in Poland. Polish Botanical Journal 61(1): 173–180
  20. Grodzki W., Starzyk J.R., Kosibowicz M., Ważny R. 2016. Parazytoidy i drapieżce owadów kambio- i ksylofagicznych oraz patogeny grzybowe na osłabionych i zamierających jodłach Abies alba Mill. w Pienińskim Parku Narodowym. Pieniny – Przyroda i Człowiek 14: 101–118
  21. Kuzniak E, Kornas A, Kazmierczak A, Rozpądek P, Nosek M, Kocurek M, Zellnig G, Muller M; Miszalski Z (2016) Photosynthesis-related characteristics of the midrib and the interveinal lamina in leaves of the C-3-CAM intermediate plant Mesembryanthemum crystallinum. Annals of Botany 117, 7: 1141-1151
  22. Slesak I, Slesak H, Zimak-Piekarczyk P, Rozpądek P (2016) Enzymatic Antioxidant Systems in Early Anaerobes: Theoretical Considerations Astrobiology 16: 5: 348-358 
  23. Ruytinx J, Elena Martino E, Rozpądek P, Daghino S, Turnau K, Colpaert J, Perotto S (2016) Homeostasis of trace elements in mycorrhizal fungi. In: Molecular Mycorrhizal Symbiosis ED Martin F; John Wiley & Sons, Inc 276-298

Patents submited

  1. Jędrzejczyk R., Jodłowski P., Chlebda D., Dziedzicka A., 2016. Sposób wytwarzania na metalicznym podłożu warstwy katalitycznego nośnika z tlenku cyrkonu(IV), nr P.418768
  2. Jodłowski P., Jędrzejczyk R., Chlebda D., Dziedzicka A., 2016. Sposób otrzymywania katalizatora tlenkowego, nr UPRP: P.417276
  3. Łojewska J., Łojewski T.,. Thomas Jacob L, Jędrzejczyk R. J., Pawcenis D., Milczarek J., Gil B., Kołodziej A., Turnau K., 2015. Modified nanocomposite material, method for its production and its application, PCT/IB2015/053408
  4. Łojewska J., Łojewski T., Thomas Jacob L., Jędrzejczyk R. J., Pawcenis D., Milczarek J., Gil B., Turnau K., Kołodziej A., 2015. Modyfikowany materiał nanokompozytowy, sposób jego wytwarzania oraz zastosowanie, zgłoszenie patentowe, a) KRAJ: nr UPRP: P.411409 b) MIĘDZYNARODOWE: [WIPO ST 10/C PL411409